our focus: 
in vivo analytic study offerings for your individual demand

axiom can provide you with analytical studies that can help you to reduce the costs of your R&D, shorten the time required for preclinical research, reduce the number of animal experiments and increase the chances of obtaining approval by Regulatory Approval. The studies are customized according to the requirements of the specific questions.

We have several applications which are technical and content driven to fit your individual need. Every customer can define its contract research order, supported by our technology team.

In doing so, we set up meetings with you to clearly understand your demand and finally receive all important parameters to create a specific proposal.

In order to estimate risks, we offer a feasibility and design study based on complex computer simulation and our experience, before we start first analytic measurments at DESY synchrotron PetraIII.

Based on your final choice of applications and focus request, we start measurement and analytics to create your data driven analysis studies.
 

Distribution Studies

  • With our analysis, you will receive extended data from ex vivo (in situ) or in vivo measurements of which entities accumulate in what quantity at which location in the entire object under investigation. 
  • Track the distribution of your drugs, drug carriers, antibodies, or immune cells over any diagnostic time window in vivo and without depth restriction in the same test object. 
  • Obtain data at different points in time on the population dynamics of tracked immune cells and visualize this information directly
  • make your biomolecules visible to imaging if it remained invisible so far

Safety Studies

  • Obtain advanced data for better risk assessment regarding the deposition and degradation of your entities linked to XFI makers for your evaluation and for exchange with Regulatory Authorities such as the FDA and EMA.

Efficacy Studies

  • Gain clarity about the achievement of objectives and efficacy of the ingredients or ingredient carrier systems 
  • Repeat in vivo measurements of efficacy in the same subject for longitudinal pharmacokinetics and pharmacodynamics purposes

If you have further requirements for analyses by axiom XFI or detailed questions about the method itself or our applications, we will be available to talk to you and your R&D team in person.
Please contact us here.

Please find below all technical applications based on axiom's advanced XFI method:

axiom in vivo small animal application

Status: Offering
Application description:

Deep in vivo tracking based on cusotmer requirements of medical drugs and/or labelled cells, e.g. T-cells in inflammatory bowel disease models.

In vivo cell tracking: axiom insights offers in vivo access to the spatio-temporal distribution of cells for monitoring cell therapies and/or immune reaction. Obtain data at different points in time on the population dynamics of tracked immune cells and visualize this information directly

In vivo medical drugs tracking: axiom insights can assess the efficacy of drugs and drug carrier by studying their pharmacokinetics and in vivo pharmacodynamics when drugs are bound to XFI markers or nanoparticles. This make your biomolecules visible to imaging if it remained invisible so far.

The animal imaging is designed in collaboration with the customer; the induced radiation dose is below damage levels, so the anesthetized test animals will still be alive after the assessment. This allows for the same animals to be used for time series studies and a reduction in the number of test animals required. We have already received the approval from the corresponding Authority for a study on in vivo cell tracking in a Crohn's Disease mouse model. Any new in vivo study needs a new approval.

Advantages:

  • Non-invasive with high spatial and temporal resolution and high sensitivity

  • Several time points over an arbitrarily long diagnostic window period in one single animal unlike ICP-MS. This reduces the number of test animals required.

  • Abritrary depth unlike optical fluorescence

axiom ex vivo (in situ) application

Status: Offering
Application description:

axiom insights provides tracking-data on distribution of molecules of interest in probes like artificial tissues and biological samples for preclinical R&D in pharma nad cosmetic industry.

Advantages: 

  • Non-invasive, hence, samples are not damaged

  • Abritrary tissue depth in contrast to laser ablation or optical fluorescence

  • High spatial resolution in contrast to Inductively Coupled Plasma – Mass Spectroscopy (ICP-MS)

axiom assays screening application

Status: Offering
Application description:

axiom insights can measure the uptake of medical drugs/ligands into cells with a very small number of cells to be used, hence lowering the pre-assay preparation work. So, axiom offers a high throughput technique, allowing the uptake of medical drugs into cells to be screened, thereby using much fewer cells than in inductively coupled plasma mass spectrometry (ICP-MS) analytics.

Advantages:

  • High throughput screening (1min per vial)

  • Low number of cells used (about 100 cells with axiom insights vs. approximately 500,000 cells with ICP-MS)

  • Uptake visible down to 0.2 picograms mass of antibody-functionalized Gold-Nano-Particles (GNP) per tumor cell

axiom feasibility & design study

Status: Offering
Application description:

We offer a simulation in order to predict the expected sensitivity and to design the optimal test setup, thereby supporting the final decision-making process

Advantages:

  • Fast feasibility check prior to beginning the major challenge of in vivo research

  • Possibility to adjust settings for improved tracking sensitivity

  • Upfront confidence with axiom insights method

axiom in vitro application

Status: Offering
Application description:

analytics of e.g. uptake of nanoparticles into cells (example: uptake of nanoparticles functionalized with antibodies by prostate tumor cells)

Advantages: 

  • Non-invasive, hence, samples are not damaged

  • Abritrary tissue depth in contrast to laser ablation or optical fluorescence

  • High spatial resolution in contrast to Inductively Coupled Plasma – Mass Spectroscopy (ICP-MS)

axiom in vivo human application

Status: R&D phase / work in progress
Application description:

in vivo tracking of medical drugs and/or labelled cells inside human patient is planned as next upgrade of axiom´s portfolio.
R&D is ongoing.